Method to export gene products to the growth medium of gram nega

Details Method to export gene products to the growth medium of gram nega Method to export gene products to the growth medium of gram nega

1990-10-24

1992-10-20

Ellis, Joan

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 91, 4351723, 4352351, 4353201, 4352523, 43525233, 536 27, 530350, 935 9, 935 27, 935 31, 935 48, 935 56, 935 58, 935 61, 935 72, 935 73, C12P 2102, C12P 1934, C12N 1500, C12N 700

Patent

active

051569590

ABSTRACT:
A process for expressing proteins in Gram(-) bacteria and providing for extracellular secretion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired protein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extracellularly secreted protein; a recombinant DNA construction comprising:

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