Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1997-07-31
2000-08-01
Schwartzman, Robert A.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
424 9321, 435455, 435456, A61K 4800, C12N 1563
Patent
active
060963034
ABSTRACT:
It has been discovered that cells such as genetically engineered fibroblasts and keratinocytes can be cultured in the cyst fluid of encapsulated tumors. This provides a means for proliferating genetically engineered producer cells within these types of tumors, increasing the number of cells producing viral particles, which then transduce the surrounding tumor cells with the genetic material, in the preferred embodiment, a lethal gene. A number of different tumor types form "cysts", which contain fluid produced by the tumor cells, including brain tumor cells such as gliomas, and many types of breast, and lung tumors. These cyst fluids have been shown to contain elevated levels of certain growth factors, for example, fibroblast growth factor (FGF) and epidermal growth factor (EGF). The types of genetically engineered cells to be used can be selected in part according to the levels of growth factors in the cyst fluid which most promote growth of the cells, for example, cystic tumors with high levels of FGF would be injected with genetically engineered fibroblasts; cystic tumors with high levels of EGF would be injected with genetically engineered keratinocytes; and cystic tumors with high levels of vascular endothelial growth factor (VEGF) would be injected with genetically engineered endothelial cells.
REFERENCES:
patent: 4352883 (1982-10-01), Lim
patent: 4868116 (1989-09-01), Morgan et al.
patent: 4980286 (1990-12-01), Morgan et al.
patent: 5558852 (1996-09-01), Bigner et al.
Gura (Nov. 1997) Systems for identifying new drugs are often faulty. Science 278:1041-1042.
Verma et al. Gene therapy--promises, problems and prospects. Nature 389:239-242, Sep. 1997.
Alkan-Onyuksel, et al., "A Mised Micellar Formulation Suitable for the Parenteral Administration of Taxol," Pharm. Res. 11(2):206-212 (1994).
Bagshawe, et al., "A cytotoxic agent can be generated selectively at cancer sites," Br. J. Cancer 58:700-703 (1988).
Bagshawe, "The First Bagshawe Lecture Towards generating cytotoxic agents at cancer cites," Br. J. Cancer 60:275-281 (1989).
Balzarini, et al., "Differential Mechanism of Cytostatic Effect of (E)-5-(2-Bromovinyl)-2'-deoxyuridine, 9-(1,3-Dihydroxy-2-propoxymethyl)guanine, and Other Antiherpetic Drugs on Tumor Cells Transfected by the Thymidine Kinase Gene of Herpes Simplex Virus Type 1 or Type 2," J. Biol. Chem. 268(9):6332-6337 (1993).
Banerji, et al., "A Lymphocyte-Specific Cellular Enhancer is Located Downstream of the Joining Region in Immunoglobulin Heavy Chain Genes," Cell 33(3):729-740 (1983).
Battelli, et al., "T lymphocyte killing by a xanthine-oxidase-containing immunotoxin," Cancer Immunol. Immunother. 35(6):421-425 (1992).
Berkner, et al., "Abundant Expression of Polyomavirus Middle T Antigen and Dihydrofolate Reductase in an Adenovirus Recombinant," J. Virology 61(4):1213-1220 (1987).
Bout, "Lung Gene Therapy: In Vivo Adenovirus-Mediated Gene Transfer to Rhesus Monkey Airway Epithelium," Human Gene Therapy 5(1):3-10 (1994).
Braunwald, et al., eds., "Principals of Cancer Therapy," in Harrison's Principles of Internal Medicine, Eleventh Edition, Chapter 79 pp. 431-446, (McGraw-Hill Book Co., 1987).
Brem, et al., "Interstitial chemotherapy with drug polymer implants for the treatment of recurrent gliomas," J. Neurosurg. 74(3):441-446 (1991).
Brem, et al., "Polymers as Controlled Drug Delivery Devices for the Treatment of Malignant Brain Tumors," Eur. J. Pharm. Biopharm. 39(1):2-7 (1993).
Brem, et al., "Intraoperative Chemotherapy Using Biodegradable Polymers: Safety and Effectiveness for Recurrent Glioma Evaluated by a Prospective, Multi-Institutional, Placebo-Controlled Clinical Trial," Proc. Amer. Clin. Oncology, p. 174, Abstract No. 487, (1994).
Brown, et al., "Review Article--Molecular and Cellular Mechanisms of Receptor-Mediated Endocytosis," DNA and Cell Biology 10(6):399-409 (1991).
Brown, et al., "Penetration of Host Cell Membranes by Adenovirus 2," J. Virology 12(2):386-396 (1973).
Bruce, et al., "Comparson of the Sensitivity of Hematopoietic Colony-Forming Cells in Different Proliferative States of 5-Fluorouracil," J. Natl. Cancer Inst. 38(3):401-405 (1967).
Caillaud, "Adenoviral Vector as a Gene Delivery System into Cultured Rat Neuronal and Glial Cells," Eur. J. Neuroscience 5(10):1287-1291 (1993).
Chardonnet, et al., "Early Events in the Interaction of Adenoviruses with HeLa Cells," Virology 40(3):462-477 (1970).
Culver, et al., "In Vivo Gene Transfer with Retroviral Vector-Producer Cells for Treatment of Experimental Brain Tumors," Science 256:1550-1552 (1992).
Davidson, et al., "Overproduction of Polyomavirus Middle T Antigen in Mammalian Cells through the Use of an Adenovirus Vector," J. Virology 61(4):1226-1239 (1987).
Fiers, et al., "Complete nucleotide sequence of SV40 DNA," Nature 273(5657):113-120 (1978).
Frattini, et al., "In Vitro synthesis of oncogenic human papillomaviruses requires episomal genomes for differentiation-dependent late expression," Proc. Natl. Acad. Sci. USA 93:3062-3067 (1996).
* Friedmann, "Overcoming the obstacles to gene therapy," Scientific American Jun.:96-101 (1997).
Gomez-Foix, "Adenovirus-mediated Transfer of the Muscle Glycogen Phosphorylase Gene into Hepatocytes Confers Altered Regulation of Glycogen Metabolism," J. Biol. Chem. 267(35):25129-25134 (1992).
Gottlieb, et al., "Treatment of Malignant Melanoma With Camptothecin (NSC-100880)," Cancer Chemother. Rep. 56(1):103-105 (1972).
Greenway, et al., "Human cytomegalovirus DNA: BamHl,-EcoRl and Pstl restriction-endonuclease cleavage maps," Gene 18:355-360 (1982).
Guzman, "Efficient Gene Transfer Into Myocardium by Direct Injection of Adenovirus Vectors," Circulation Research 73(6):1201-1207 (1993).
Haj-Ahmad, et al., "Development of a Helper-Independent Human Adenovirus Vector and Its Use in the Transfer of the Herpes Simplex Virus Thymidine Kinase Gene," J. Virology 57(1):267-274 (1986).
Hughes, et al., "Monoclonal Antibody Targeting of Liposomes to Mouse Lung in Vivo," Cancer Research 49(22):6214-6220 (1989).
Kirshenbaum, "Highly Efficient Gene Transfer into Adult Ventricular Myocytes by Recombinant Adenovirus," J. Clin. Invest. 92:381-387 (1993).
La Salle, "An Adenovirus Vector for Gene Transfer into Neurons and Glia in the Brain" Science 259:988-990 (1993).
Litzinger, et al., "Biodistribution and immunotargetability of ganglioside-stabilized dioleoylphosphatidylethanolamine liposomes," Boichimica et Biophysica Acta 1104(1):179-187 (1992).
Lusky, et al., "Bovine Papilloma Virus Contains an Activator of Gene Expression at the Distal End of the Early Transcription Unit," Mol. Cell Bio. 3(6):1108-1122 (1983).
Massie, et al., "Construction of a Helper-Free Recombinant Adenovirus That Expresses Polyomavirus Large T Antigen," Mol. Cell. Biol. 6(8):2872-2883 (1986).
Matsuda, et al., "Photoinduced Prevention of Tissue Adhesion," ASAIO Trans. 38(1):154-157 (1992).
Moertel, et al., "Phase II Study of Camptothecin (NSC-100880) in the Treatment of Advanced Gastrointestinal Cancer," Cancer Chemother. Rep. 56(1):95-101 (1972).
Morsy, "Efficient Adenoviral-mediated Ornithine Transcarbamylase Expression in Deficient Mouse and Human Hepatocytes," J. Clin. Invest. 92(3):1580-1586 (1993).
Moullier, "Correction of lysosmal storage in the liver and spleen of MPS VII mice by implantation of genetically modified skin fibroblasts," Nature Genetics 4(2):154-159 (1993).
Muggia, et al., "Phase I Clinical Trial of Weekly and Daily Treatment With Camptothecin (NSC-100880): Correlation With Preclinical Studies," Cancer Chemother. Rep. 56(4):515-521 (1972).
Mullen, et al., "Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: A negative selection system," Proc. Natl. Acad. Sci. USA 89(1):33-37 (1992).
Mulligan, et al., "Expression of a Bacterial Gene in Mammalian Cells," Science 209(4463):1422-1427 (1980).
Mulligan, et al., "The Basic Science of Gene Therapy," Science 260:926-932 (1993).
* Oldfield, et al., "Gene therapy for the treatment of brain tumors using intra-tumoral transduction with the thymidine gene and intraveno
Medical College of Georgia Research Institute Inc.
Schwartzman Robert A.
LandOfFree
Method to enhance treatment of cystic tumors does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method to enhance treatment of cystic tumors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method to enhance treatment of cystic tumors will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-660967