Method to directly radiolabel antibodies for diagnostic imaging

Details Method to directly radiolabel antibodies for diagnostic imaging Method to directly radiolabel antibodies for diagnostic imaging

1990-03-27

1991-04-30

Maples, John S.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

530388, 530389, 530402, A61K 39395

Patent

active

050116762

ABSTRACT:
The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.

REFERENCES:
patent: 4305922 (1981-12-01), Rhodes
patent: 4401646 (1983-08-01), Rhodes et al.
patent: 4416865 (1983-11-01), Rhodes et al.
patent: 4424200 (1984-01-01), Crockford et al.
patent: 4472371 (1984-09-01), Burchiel et al.
patent: 4478815 (1984-10-01), Burchiel et al.
patent: 4645660 (1987-02-01), Takahashi et al.
patent: 4652400 (1987-03-01), Paik et al.
patent: 4837003 (1989-06-01), Nicolotti
patent: 4851515 (1989-07-01), Bonnyman et al.
Khaw, B. A., Strauss, W. and Carvalho, A. et al., Tc-99m Labeling of Antibodies to Cardiac Myosin Fab and to Human Fibrinogen, J. Nucl. Med. 23, 1011-109, 1982.
Paik, C. H., Pham, L. N. B., Hong, J. J. et al., The Labeling of High Affinity Sites of Antibodies with Tc-99m, Int. J. Nucl. Med. and Biol. 12, 3-8, 1985.
Thakur, M. L., Richard, M. D., and White, F. W., III, Monoclonal Antibodies as Agents for Selective Radiolabeling of Human Neutrophils, J. Nucl. Med. 29, 1817-1825, 1988.
Franz, J., Volkert, W. A., Barefield, E. K., et al. The Production of Tc-99m Labeled Conjugated Antibodies Using a Cyclam-Based Bifunctional Chelating Agent, Int. J. Nucl. Med. Biol. 14, 569-572, 1987.
Fritzber, A. R., Kasina, S., Reno, J. M. et al., Radiolabeling Antibodies Using N.sub.2 S.sub.2 Ligands, J. Nucl. Med. 27, 957-958, 1986.
Lin et al., Use of Fe(II) or Sn(II) Alone for Technetium Labeling of Albumin, J. Nucl. med., vol. 12, No. 5, pp. 204-210.
Eckelman et al., .sup.99m Tc-Human Serum Albumin, J. Nucl. Med., vol. 12, No. 11, 707-710 (1971).
Pettit Improved Protein Labeling by Stannous Tartrate Reduction of Pertechnetate, J. Nucl. Med., vol. 21, No. 1, pp. 59-62 (1980).
Huang et al. Detection of Bacterial Endocarditis with Technetium-99m-Labeled Antistaphyloccoccal Antibody, J. Nucl. Med., vol. 21, No. 8, pp. 783-786 (1980).
Rhodes et al., Technetium-99m Labeling of Murine Monoclonal Antibody Fragments, J. Nucl. Med., vol. 27, No. 5, pp. 685-693 (1986).
Sundrehagen et al., Formation of .sup.99m Tc-Immunoglobulin G Complexes Free from Radiocolloids, Quality Controlled by Radioimmunoelectrophoresis, European J. Nucl. Med., vol. 7, pp. 549-552 (Spring 1982).
Blok et al., A New Method for Protein Labeling with .sup.99m Tc, Nucl. Med. Biol., vol. 16, No. 1, pp. 11-16 (1989).
Stiegman et al., The Importance of the Protein Sulfhydryl Group in HSA Labeling with Technetium-99m, Proceedings of 22nd Annual Meeting, J. Nucl. Med., vol. 16, No. 6.
Liang et al., Serum Stability and Non-Specific Binding of Technetium-99m Labeled Diaminodithiol for Protein Labeling, Nucl. Med. Biol., vol. 14, No. 6, pp. 555-561 (1987).
Schwartz, A. and Steinstrabber, A., A Novel Approach to Tc-99m Labeled Monoclonal Antibodies, J. Nucl. Med. 28, 721, 1987.
Pak et al., A Rapid and Efficient Method for Labeling IgG Antibodies with Tc-99m and Comparison to Tc-99m FAB' Antibody Fragments, J. Nucl. Med., vol. 30, p. 793 (1989).
Del Rosario et al., Site-Specific Radiolabeling of Monoclonal Antibodies with Biotin/Streptavidin, Nucl. Med. Biol., vol. 16, pp. 525-527 (1989).
Cleland, Dithiothreitol, A New Protective Reagent for SH Groups, Biochemistry, vol. 3, pp. 480-482, (1986).
Hawkins et al., Resistance of Direct Tc-99m-Protein Bond to Transchelation, Antibody, Immunoconjugates, and Radiopharmaceuticals, vol. 3, No. 1 (1990).
Jones, A. G., Orvig, C., Trop, H. S. et al., A Survey of Reducing Agents for the Synthesis of Tetraphenylarsoniumoxotechnetium (Ethanedithiolate) from Tc-99m Pertechnetate in Aqueous Solution, J. Nucl

Affiliated with

Also associated with

Rating

Method to directly radiolabel antibodies for diagnostic imaging does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method to directly radiolabel antibodies for diagnostic imaging , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method to directly radiolabel antibodies for diagnostic imaging will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-639458