Method to determine automatically the sequence of amino acids

Details Method to determine automatically the sequence of amino acids Method to determine automatically the sequence of amino acids

1974-02-15

1976-05-25

Love, Ethel G.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

23230M, 23252R, C07D 4932

Patent

active

039593070

ABSTRACT:
Method and apparatus for automatically converting of thiazolinone amino acids into phenyl thiohydantoin amino acids as a stage in amino acid sequence analysis of proteins and peptides. According to the invention a solution of thiazolinone amino acids is, under continuous exclusion of oxygen, introduced into a reaction vessel and evaporated to dryness. Then there is added an aqueous trifluoro acetic acid solution or dilute hydrochloric acid, containing a mercaptan, and again evaporated, followed by dissolving of the residue in a mixture of dichloroethane and methanol.

REFERENCES:
edman, P., Acta Chem. Scand. 4, (1950), pp. 277-282.
Ilse et al., Austral. Journ. Chem. 16, (1963), pp. 411-416.
Edman, P., Acta Chem. Scand. 10 (1956), pp. 761-768.
Wittman-Liebold, Hoppe-Seylers Zeitschrift fuer Physiologische Chemie, Bd. 354, pp. 1415-1431, 10-11/1973.
Edman et al., European Journal of Biochemistry, vol. 1, pp. 80-91 (1967).
Edman, Royal Australian Chemical Institute, Proceedings, 1957.

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