Method to detect protein-protein interactions

Details Method to detect protein-protein interactions Method to detect protein-protein interactions

1995-05-04

1997-06-10

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435697, 4351721, 4353201, 530350, 536 2301, 536 235, C12Q 108, C12P 2100, C07K 100, C07H 2104

Patent

active

056374631

ABSTRACT:
Methods are provided for studying protein-protein interactions which require posttranslational modification of one of the proteins. The interaction is detected by reconstituting the activity of a transcriptional activator. This activity is dependent on the interactions between three different proteins. These include two chimeric proteins, one of which must be posttranslationally modified by the activity of the third protein in order for the chimeric proteins to interact. One of the chimeric proteins contains a transcriptional activation domain fused to a test protein. The second chimeric protein contains a DNA-binding domain of a transcriptional activator fused to the other test protein.

REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
patent: 5503977 (1996-04-01), Johnsson et al.
Luban et al. The yeast two-hybrid system for studying protein-protein interactions Current Opinion in Biotechnology vol. 6 59-64 1995.
Keegan et al., Science 231:699-704 (1986).
Curran et al., Cell, 55:395-397 (1988).
McKnight et al., Proc. Natl. Acad. Sci. USA, 84:7061-7065 (1987).
Paolini, R., et al., J. Exp. Med. 181:247-255 (1995).
Kihara, H., et al., J. Biol. Chem., 269:22427-22432 (1994).
Shiue, L., et al., Mol. Cell. Biol., 15:272-281 (1995).
Brent & Ptashne, Cell, 43:729-736 (1985).
Dalton and Treisman, Cell, 68:597-612 (1992).
Fields and Sternglanz, Trends Genet., 10:286-292 (1994).

Affiliated with

Also associated with

Rating

Method to detect protein-protein interactions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method to detect protein-protein interactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method to detect protein-protein interactions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-763843