Method to detect hormone treatment in animals

Chemistry: analytical and immunological testing – Food or dairy products – Dairy product

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436 20, 436 22, 436 86, 436 87, 424535, G01N 3306

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active

056354010

ABSTRACT:
The invention relates to measuring the decrease in phosphorylation of the fatty acid binding protein (FABP) found in the milk fat globule membrane (MFGM) of bovine or human milk to detect growth hormone treatment in animals. MFGM of cows treated with recombinan+bovine somatotropin (bST) displays weaker FABP autophosphorylation activity than non treated cows. MFGM isolated from cows treated with bST have significantly reduced levels of phosphorylated FABP. The bST test of the present invention can be used not only to determine whether the animal producing the milk has been treated with bST, it can also be used to determine the efficacy of bST on milk production. Dairy managers could thus base their decision on whether to continue bST treatment on such a test.

REFERENCES:
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Spitsberg, V.L. et al, Phosphorylation of Bovine and Human Milk Fat Globule Membrane (MFGM) and Bovine Skim Milk Membrane (SMM) Proteins: Effect of Metals, Inhibitors, and Troton X-100, The FASEB Jounal, Abstracats Part I, Mar. 9, 1995, vol. 9, No. 3.
Matitashvili, E. Et al, Effect of Mammary Derived Growth Inhibitor (MDGI/FABP), Isolated from Bovine Milk, on MAC-T and primary mammary epithelial cells: Comparison with a Synthetic MDGI/FABP Derivative, J. Anam Sci. vol. 77, Suppl. 1, (1994).
Spitsberg, V.T. et al, Predicting Producation Responses of Cows Receiving bST using Milk Fat, Dairy Management, Inc., Research Funding Application, Jul. 1995.
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