Method to detect fertilization potential of sperm

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

Reexamination Certificate

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C435S002000, C435S806000

Reexamination Certificate

active

06884601

ABSTRACT:
The invention relates to a method to detect male antifertility problems based on the determination of latent phospholipid hydroperoxide glutathione peroxidase (PHGPx).

REFERENCES:
patent: 5895749 (1999-04-01), Alvarez
patent: 6231853 (2001-05-01), Hillman et al.
patent: WO 9613225 (1996-05-01), None
Maiorino M. Testosterone Mediates Expression of the Selenoprotein PHGPx . . . The FASEB J, vol. 12, 1359-1170, 1998.*
Ursini F. Dual Function of the Selenoprotein PHGPx During Sperm Maturation. Science 285 1393-1396, 1999.*
Maurer S. Attempt to Differentiate Between Individual Glutathione Peroxidases in Biological Samples. Z Ernahrungswiss 37 Supp 1, 110-113, 1998.*
Roveri A. Enzymatic and Immunological Measurements of Soluble and Membrane Bound PHGP. Methods in Enzymology 233, 202-212, 1994.*
Maiorino M. Phospholipid Hydroperoxide Glutathione Peroxidase. Methods in Enzymology 184, 448-457, 1990.*
Roveri A. Purification and Characterization of Phospholipid Hydroperoxide Glutathione Peroxidase from Rat Testis Mitochondrial Membranes. Biochimica et Biophysica acta 1208(2)211-221, 1994.*
Godeas C. Distribution and Possible Novel Role of Phospholipid Hydroperoxide Glutathione Peroixdase in Rat Epidiymal Spermatozoa. Biology of Reproduction 57(6)1502-1508, 1997.

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