Method to collect and recover microorganisms from environmental

Details Method to collect and recover microorganisms from environmental Method to collect and recover microorganisms from environmental

1998-07-23

2000-01-04

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 30, 4352886, 4353081, 210696, 210697, 209 5, 423314, C12Q 124, B03D 300, C12N 102, C01B 1516

Patent

active

06010869&

ABSTRACT:
Presented herein is an improved method to collect and recover microorganisms from environmental samples. The method of this invention comprises the steps of (a) obtaining a sample for testing and, optionally, suspending the sample in a suitable liquid, (b) amending the sample with sodium hexametaphosphate and, optionally, centrifuging the sample to remove solid sediments, insoluble salts, inert materials, and the like, (c) centrifuging the sample through a separation column amended with sodium hexametaphosphate, and (d) recovering the microorganism-containing material remaining above the column for analysis.

REFERENCES:
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Taylor et al. Microscope, 28, pp. 47-49. (1980).
Holben et al. Applied and Environmental Microbiology, 54 (3), pp. 703-711. (Mar. 1988).
Katayama et al. Soil Science and Plant Nutrition, 44 (2), pp. 245-252. (Jun. 1998).

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