Method to achieve a linear standard curve in a sandwich immunoas

Details Method to achieve a linear standard curve in a sandwich immunoas Method to achieve a linear standard curve in a sandwich immunoas

1987-04-30

1988-11-29

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436513, 436533, 436534, 436801, 436803, 436817, 436820, 436827, G01N 33532, G01N 33546, G01N 33574, G01N 33576

Patent

active

047881380

ABSTRACT:
A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody:antigen:labelled antibody complex which is then detected. Unbound unlabelled first antibody and/or unbound unlabelled second antibody are added to the reaction mixture to divert excess antigen away from the desired end-product complex, thus rendering the antigen of interest the rate-limiting factor in the overall immunoreaction. This results in a pseudo first-order reaction which produces an actual linear standard curve.

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