Method test media and chromogenic compounds for identifying and

Details Method test media and chromogenic compounds for identifying and Method test media and chromogenic compounds for identifying and

1990-04-20

1993-05-11

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 71, 435 737, C12Q 104, C12Q 102, C12Q 100, G01N 3353

Patent

active

052100227

ABSTRACT:
A chromogenic .beta.-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by .beta.-galactosidase and a chromogenic .beta.-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by .beta.-glucuronidase are combined in a test medium for quantitatively identifying and differentiating general coliforms and E. coli. The .beta.-galactosidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by .beta.-galactosidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-glucuronide, 4,6-dichloroindolyl-.beta.-D-glucuronide, 6,7-dichloroindolyl-.beta.-D-glucuronide, and 4,6,7-trichloroindolyl-.beta.-D-glucuronide, which produce mauve or magenta precipitates when reacted upon by .beta.-glucuronidase. The .beta.-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-glucuronide, which produces an indigo blue precipitate when reacted upon by .beta.-glucuronidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-galactoside, 4,6-dichloroindolyl-.beta.-D-galactoside, 6,7-dichloroindolyl-.beta.-D-galactoside, and 4,6,7-trichloroindolyl-.beta.-D-galactoside which produce mauve or magenta precipitates when reacted upon by .beta.-galactosidase.

REFERENCES:
patent: 3496066 (1970-02-01), Berger et al.
patent: 3870601 (1975-03-01), Warren et al.
patent: 3936356 (1976-02-01), Janin
patent: 3957584 (1976-05-01), Kronish
patent: 4351823 (1982-09-01), Rubin
patent: 4556636 (1985-12-01), Belly et al.
patent: 4591554 (1986-05-01), Koumura et al.
patent: 4603108 (1986-07-01), Bascomb
patent: 4673638 (1987-06-01), Grosch et al.
Watkins et al., Appl. Environ. Microbiol., 54:1874-1875 (1988).
Ley et al., Can. J. Microbiol., 34:690-693 (1987).
Sadler et al., J. Am. Chem. Soc., 78:1251-1255 (1955).
Sales Brochure of Access Medical Systems, Inc., Cat. #IS-001 Jan. 1988 for Colilert MPN.
"Studies in Enzyme Cytochemistry III. Relationships Between Solubility, Molecular Association and Structure in Indigoid Dyes", Holt et al, Proc. Royal Soc. London, vol. 148 B, pp. 495-505 (1958).

Affiliated with

Also associated with

Rating

Method test media and chromogenic compounds for identifying and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method test media and chromogenic compounds for identifying and , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method test media and chromogenic compounds for identifying and will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1349619