Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1993-06-03
1994-10-25
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 34, 435 39, 435 38, 435 21, 536 174, 536 173, 536 181, 536 41, C12Q 104, C12Q 106, C12Q 110, C07H 1500
Patent
active
053588541
ABSTRACT:
A chromogenic .beta.-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by .beta.-galactosidase and a chromogenic .beta.-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by .beta.-glucuronidase are combined in test medium for quantitatively identifying and differentiating general coliforms and E. coli. The .beta.-galactosidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by .beta.-galactosidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-glucuronide, 4,6-dichloroindolyl-.beta.-D-glucuronide, 6,7-dichloroindolyl-.beta.-D-glucuronide, and 4,6,7-trichloroindolyl-.beta.-D-glucuronide, which produce mauve or magenta precipitates when reacted upon by .beta.-glucuronidase. The .beta.-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-glucuronide, which produces an indigo blue precipitate when reacted upon by .beta.-glucuronidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-galactoside, 4,6-dichloroindolyl-.beta.-D-galactoside, 6,7-dichloroindolyl-.beta.-D-galactoside, and 4,6,7-trichloroindolyl-.beta.-D-galactoside which produce mauve or magenta precipitates when reacted upon by .beta.-galactosidase.
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Leary Louise N.
Research Organics, Inc.
Wityshyn Michael G.
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