Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1991-10-22
1994-07-26
Yarbrough, Amelia Burgess
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435968, 436 56, 436805, 935 77, C12P 1934
Patent
active
053326660
ABSTRACT:
A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments. The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine. The fluorescent dye preferably is attached to the DNA chain terminators and provides many advantages. Thus only DNA sequencing fragments resulting from bona fide termination events will carry a reporter. The DNA sequencing may also be labeled using the xanthene dyes which have general utility as fluorescent labels. Also acyclonucleoside triphosphates are described as being useful as chain terminators in DNA sequencing using a modification of the Sanger method.
REFERENCES:
patent: 4855225 (1989-08-01), Fung et al.
A. M. Maxam et al., Methods in Enzym., Sequencing End-Labeled DNA with Base-Specific Chemical Cleavages', 1980, vol. 65, pp. 499-559.
F. Sanger et al., Proc. Nat. Acad. Sci., DNA Sequencing with chain-terminating inhibitors, Oct. 3, 1977, vol. 74, pp. 5463-5467.
Lloyd, M. Smith et al., Nucleic Acids Research, vol. 13, Mar. 19, 1985, pp. 2399-2412.
Naimski et al., 1980, vol. 106, Anal. Biochem. pp. 471-475.
Chidgeavadge, Z. G. et al., Nucl. Acids. Res., vol. 12, pp. 1671-1686, 1984.
Krayevsky A. et al., Nucl. Acids. Res., Symp. Series No. 1671-1686, 1984. pp. 283-284.
Robert S. Beabealashvilli et al., Biochim. Biophys. Acta., vol.l 868, 1986, pp. 136-144.
Zurab G. Chidgeavadze et al., Biochim. Biophys. Acta, 1986, pp. 145-162, vol. 868.
Bergstrom et al., J. Am. Chem. Soc., vol. 98, pp. 1587, 1976.
Ruth, PCT/US84/00279.
Langer et al., Proc. Natl. Acad. Sci. USA, vol. 78, No. 11, pp. 6633-6637, 1981.
Draper, Nucleic Acids Research, vol. 12, No. 2, pp. 989-1002, 1984.
Barr et al, J. Chem. Soc., Perkins Trans. I, pp. 1263-1267, 1978.
Bergstrom et al., J. Am. Chem. Soc., vol. 100, pp. 8106, 1978.
Vincent et al., Tetrahedron Letters, vol. 22, pp. 945-947, 1981.
Robins et al., J. Org. Chem., vol. 48, pp. 1854.varies.1862, 1983.
Sonograshira et al., Tetrahedron Letters, No. 50, pp. 4467-4470, 1975.
Edo et al., Chem. Pharm. Bull., vol. 26, No. 12, pp. 3843-3850, 1978.
Seela et al., Chem. Ber., vol. 111, pp. 2925-2930, 1978.
Schram et al., J. Carbohydrates, Nucleosides and Nucleotides, vol. 2, No. 2, pp. 177-184, 1975.
Bergstrom et al., J. Org. Chem., vol. 46, No. 7, pp. 1423-1431, 1981.
Bergstrom et al., Nucleic Acids Research, vol. 8, pp. 6213-6219, 1980.
Haralambidis et al., Nucleic Acids Research, vol. 15, No. 12, pp. 4857-4876, 1987.
Gibson et al., Nucleic Acids Research, vol. 15, No. 16, pp. 6455-6467, 1987.
Dam Rudy J.
Hobbs, Jr. Frank W.
Prober James M.
Robertson, Jr. Charles W.
Trainor George L.
Burgess Yarbrough Amelia
E. I. Du Pont de Nemours and Company
LandOfFree
Method, system and reagents for DNA sequencing does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method, system and reagents for DNA sequencing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method, system and reagents for DNA sequencing will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1052638