Optics: measuring and testing – Sample – specimen – or standard holder or support – Fluid containers
Reexamination Certificate
2008-01-22
2010-06-15
Punnoose, Roy (Department: 2886)
Optics: measuring and testing
Sample, specimen, or standard holder or support
Fluid containers
C356S039000
Reexamination Certificate
active
07738094
ABSTRACT:
The present invention provides a low cost imaged-based system for detecting, measuring and/or counting labeled features of biological samples, particularly blood specimens. In one aspect, the invention includes a system for imaging multiple features of a specimen that includes one or more light sources capable of successively generating illumination beams each having a distinct wavelength band and a plurality of differentially excitable labels capable of labeling a specimen comprising multiple features, such that each different feature is labeled with a different differentially excitable label. System of the invention may further include a controller operationally associated with the one or more light sources for successively directing illumination beams onto the specimen so that each of the different differentially excitable labels is successively caused to emit an optical signal within the same wavelength band, an optical system capable of collecting such emitted optical signals and forming successive images corresponding to the labeled features of the specimen on a light-responsive surface to form successive sets of image data thereof, and a disposable cuvette for collection and optical analysis of non-red blood cells.
REFERENCES:
patent: 3819913 (1974-06-01), Carter et al.
patent: 3916205 (1975-10-01), Kleinerman
patent: 3963350 (1976-06-01), Watanabe
patent: 4088448 (1978-05-01), Lilja et al.
patent: 4125828 (1978-11-01), Resnick et al.
patent: 4133873 (1979-01-01), Noller
patent: 4337222 (1982-06-01), Kitajima et al.
patent: 4501496 (1985-02-01), Griffin
patent: 4727020 (1988-02-01), Recktenwald
patent: 4751188 (1988-06-01), Valet
patent: 4857735 (1989-08-01), Noller
patent: 5053626 (1991-10-01), Tillotson
patent: 5073857 (1991-12-01), Peters et al.
patent: 5102625 (1992-04-01), Milo
patent: 5134662 (1992-07-01), Bacus et al.
patent: 5159642 (1992-10-01), Kosaka
patent: 5187749 (1993-02-01), Sugimoto
patent: 5196709 (1993-03-01), Berndt
patent: 5200152 (1993-04-01), Brown
patent: 5294799 (1994-03-01), Aslund et al.
patent: 5332905 (1994-07-01), Brooker et al.
patent: 5348859 (1994-09-01), Brunhouse et al.
patent: 5385539 (1995-01-01), Maynard
patent: 5489771 (1996-02-01), Beach et al.
patent: 5491343 (1996-02-01), Brooker
patent: 5547849 (1996-08-01), Baer et al.
patent: 5674457 (1997-10-01), Williamsson et al.
patent: 5732150 (1998-03-01), Zhou et al.
patent: 5733721 (1998-03-01), Hemstreet et al.
patent: 5851835 (1998-12-01), Groner
patent: 6154282 (2000-11-01), Lilge et al.
patent: 6159740 (2000-12-01), Hudson et al.
patent: 6350613 (2002-02-01), Wardlaw et al.
patent: 6453060 (2002-09-01), Riley et al.
patent: 6563585 (2003-05-01), Rao et al.
patent: 6594075 (2003-07-01), Kanao
patent: 6612111 (2003-09-01), Hodges
patent: 6638769 (2003-10-01), Lilja et al.
patent: 6665060 (2003-12-01), Zahniser et al.
patent: 6716588 (2004-04-01), Sammak et al.
patent: 6723290 (2004-04-01), Wardlaw
patent: 6825921 (2004-11-01), Modlin et al.
patent: 6828567 (2004-12-01), Amirkhanian et al.
patent: 6831733 (2004-12-01), Pettersson et al.
patent: 6869570 (2005-03-01), Wardlaw
patent: 6985224 (2006-01-01), Hart
patent: 7094562 (2006-08-01), Bittner
patent: 7133545 (2006-11-01), Douglass et al.
patent: 7146372 (2006-12-01), Bacus et al.
patent: 7149332 (2006-12-01), Bacus et al.
patent: 2003/0170613 (2003-09-01), Straus
patent: 2005/0190058 (2005-09-01), Call
patent: 2006/0024756 (2006-02-01), Tibbe et al.
patent: 2007/0178009 (2007-08-01), Sakaino et al.
patent: 2007051861 (2007-05-01), None
Beach J. M. “A LED light calibration source for dual-wavelength microscopy,” Cell Calcium, 21 (1): 63-68 (1997).
Chang et al, “A microfluidic device for practical label-free CD4 + T cell counting of HIV—infected subjects,” Lab Chip, 7: 170-178 (2007).
DeBernardi et al, “Single cell Ca2+/cAMP cross-talk monitoring by simutaneous Ca2+/cAMP fluorescence ratio imaging,” Proc. Natl. Acad. Sci. 93:4577-4582 (1996).
Fischer et al, “An affordable, portable fluorescent imaging device for skin lesion detection using a dual wavelength approach for image contrast enhancement and aminolaevulinic acid-induced protoporphyrin IX. Part I. Design, spectral and spatial characteristics,” Lasers Med Sci., 16: 199-296 (2001).
Fischer et al, “An affordable, portable fluorescent imaging device for skin lesion detection using a dual wavelength approach for image contrast enhancement and aminolaevulinic acid-induced protoporphyrin IX. Part II. In vivo testing,” Lasers Med Sci., 16: 207-212 (2001).
Takashi Fukano et al, “Fast dual-excitation radiometry with light-emitting diodes and high-speed liquid crystal shutters,” Biochemical and Biophysical Research Communications, 340:250-255 (2006).
Gerstner et al, “Quantitative Histology by Multicolor Slide-based Cytometry,” Cytometry Part A, 50A: 210-219 (2004).
Hart et al, “Light emitting diode excitation emission matrix fluorescence spectroscopy,” Anlayst. (127): 1693-1699 (2002).
Heiden et al, “New Epi-Fluorescence optical system for independent analysis of two different fluorochromes in microscopy,” Cytometry 20: 95-101 (1995).
Holland et al, “Point-of-care molecular diagnostic systems—past, present and future,” Current Opinion in Microbiology, 8: 504-509 (2005).
Janossy et al, “Precise CD4 T-Cell counting using red diode laser excitation: for richer, for poorer,” Cytometry (Clinical Cytometry) 50: 78-85 (2002).
Kassotis et al, “An inexpensive dual-excitation apparatus for fluorescence microscopy,” Pfugers Arch., 409: 47-51 (1987).
Li et al, “CD4+T lymphocytes enumeration by an easy-to-use single platform Image cytometer for HIV monitoring in resource-constrained settings,” Cytometry Part B (Clinical Cytometry) 728: 397-407 (2007).
Rodriguez et al, “A microchip CD$ counting method for HIV monitoring in resource-poor settings,” PLoS Medicine, 2 (7): 0663-0672 (2005).
Sapiro, “Cellular astronomy—a foreseeable future in cytometry,” Cytometry Part A, 60A: 115-124 (2004).
Sapiro, “Personal cytometers: Slow flow or no flow,” Cytometry Part A, 69A: 620-630 (2006).
Tsien et al, “Measurement of cytosolic free Ca2+in individual small cells using fluorescence microscopy with dual excitation wavelengths,” Cell Calcium 6: 145-157 (1985).
Toner et al, “Blood-on-a-chip,” Annu. Rev. Biomed. Eng. 7: 77-103 (2005).
Warner et al, “Multicomponent analysis in clinical chemistry by use of rapid scanning fluorescence spectroscopy,” Clin. Chem. 22/9: 1483-1492 (1967).
Wittrup et al, “Fluorescence array detector for large-field quantative fluorescence cytometry,” Cytometry 16: 206-213 (1994).
Yager et al, “Microfluidic diagnostic technologies for global public health,” Nature 442: 412-418 (2006).
Ymeti et al, “A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection,” Cytometry Part A 71A: 132-142 (2007).
Lewis, Ernest K. et al., “Color-blind fluorescence detection for four-color DNA sequencing”; PNAS; www.pnas.org/cgi/doi/10/1073/pnas.0501606102; Apr. 12, 2005; vol. 102, No. 5: 5346-5351.
Becton Dickinson and Company
Bozicevic Field & Francis LLP
Punnoose Roy
Scherer David C.
LandOfFree
Method, system, and compositions for cell counting and analysis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method, system, and compositions for cell counting and analysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method, system, and compositions for cell counting and analysis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4246629