Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-07-27
1994-11-29
Russel, Jeffrey E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 10, 435 19, 435 23, 435810, C12Q 130, C12Q 162, C12Q 144
Patent
active
053690133
ABSTRACT:
A method, reagent mixture and test kit are provided for testing a urine sample for the presence of bacterial or somatic cells, by reacting a urine sample with a catalase-free alkaline protease enzyme and a detergent compatible therewith, so as to disrupt any such cells present in the sample and release active catalase therefrom. The presence of catalase is detected by the formation of foam upon the addition of H.sub.2 O.sub.2. The reaction of the sample with the protease enzyme and the detergent is conducted in the presence of: (a) a buffer providing for a pH of about 8.5 to about 9; and optionally (b) one or more additional solutes; the total concentration of components (a) and (b), when present, being from about 0.02M to about 0.4M.
REFERENCES:
patent: 3764479 (1973-10-01), Bergeron
patent: 3853471 (1974-12-01), Rittersdorf
patent: 3853472 (1974-12-01), Rittersdorf
patent: 4116773 (1978-09-01), Polito
patent: 4311794 (1982-01-01), Melnick et al.
patent: 4314030 (1982-02-01), Habich
patent: 4481294 (1984-11-01), Downs
Methods of Enzymatic Analysis, Third Edition,(Ed.-in-chief: H. V. Bergmey Verlag Chemie GmbH, Weinheim FRG (1983), vol. II, pp. 26-83.
Gitomer Ralph
Russel Jeffrey E.
Yissum Research Development Company of the Hebrew University of
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