Method of washing blood cells and container assembly thereof

Imperforate bowl: centrifugal separators – Process

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422 72, 494 27, 494 45, B04B 502, B04B 1106

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active

051143968

ABSTRACT:
Thawed glycerolized red blood cells are washed in a system (1) of closed collapsible containers of flexible material which are positioned concentrically in a centrifuge rotor. The blood cells are held in an annular primary container (2) into which wash liquid is centrifugally fed from a c entral container (3) and from which supernatant is expressed into a central waste container (4) while the primary container is being compressed as a result of centrifugal action on an elastic body (24) in the rotor. A container assembly (1) for use in carrying out the washing comprises an annular collapsible primary container (2), a collapsible circular closed wash liquid container (3), a collapsible circular, closed waste container (4), and valve controlled conduits for passing liquid from the wash liquid container into the primary container and from the primary container into the waste container. The wash liquid container (3) and the waste container (4) are positioned one on top of the other in the circular area surrounded by the primary container (2).

REFERENCES:
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patent: 3679128 (1972-07-01), Unger et al.
patent: 3737096 (1973-06-01), Jones et al.
patent: 3864089 (1975-02-01), Tiffany et al.
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Transfusion, vol. 12, No. 4, pp. 237-244, Jul.-Aug. 1972 (A. H. Runck et al) "Continuous flow Centrifugation Washing of Red Blood Cells".
Transfusion, vol. 16, No. 6, Nov.-Dec. 1976, (T. J. Contreras et al) "A Comparison of Methods to Wash Liquid-Stored Red Blood Cells and Red Blood Cells Frozen with High or Low Concentrations of Glycerol", pp. 539-565.

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