Method of visually demonstrating the presence of microorganisms,

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a...

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435 29, 435 4, 435863, 435864, 435865, 435866, C12Q 118, C12Q 102

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055232140

ABSTRACT:
A method of visually demonstrating the growth of such microorganisms as fungi, yeasts, and bacteria, especially mycobacteria, identifying them, and testing them for sensitivity to antibiotics by the color change of a specific redox indicator system. A mixture of the redox indicators Methylene Blue and resazurin is added to the culture medium. Iron(III) salts mixed with K.sub.3 Fe(CN).sub.6, Iron(II) salts mixed with K.sub.4 Fe(CN).sub.6, or sodium tungstate (Na.sub.2 WO.sub.4) is added to the culture medium.

REFERENCES:
patent: 5126247 (1992-06-01), Palmer et al.
patent: 5316918 (1994-05-01), Ollar
C. Ramsdell et al., J. Dairy Sci., vol. XVIII, No. 11, pp. 705-717, 1935.
Mundinger et al., Molkerei Zeitung, vol. 47(9), pp. 171-174, 1933.
The Merch Index, Eleventh Edition, 1989, pp. 954, 894, 1433.

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