Method of using synthesis of 1,2-dioxetanes and kits therefore

Details Method of using synthesis of 1,2-dioxetanes and kits therefore Method of using synthesis of 1,2-dioxetanes and kits therefore

1997-06-25

1999-01-05

Owens, Amelia

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

549332, 5303871, 536 243, 435 4, 435 76, 435 772, C07F 928, C12Q 100, G01N 3353

Patent

active

058565221

ABSTRACT:
Kits comprising 1,2-dioxetanes which can be cause to chemiluminesce by contact with an enzyme, and the enzyme, are provided for use in optically detectable assays. The assay calls for binding the enzyme to the substance to be detected in a sample, removing any unbound enzyme, and then combining the treated sample with the dioxetane. If the substance to be detected is present, enzyme bound thereto will cleave the protecting group of the dioxetane, causing the dioxetane to decompose and chemiluminescence. The intensity of luminescence is indicative of the concentration of the substance in the sample. The substance to be detected may be an enzyme, in which case no binding group is necessary.

REFERENCES:
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patent: 5340716 (1994-08-01), Ullman et al.
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patent: 5709994 (1998-01-01), Pease et al.
patent: 5721370 (1998-02-01), Akhavan et al.
patent: 5777133 (1998-07-01), Bronstein et al.
patent: 5777135 (1998-07-01), Akhavan et al.

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