Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Reexamination Certificate
2003-11-10
2008-08-05
Noguerola, Alex (Department: 1795)
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
C427S230000, C427S235000, C210S656000
Reexamination Certificate
active
07407568
ABSTRACT:
A novel on-line method is presented for the extraction and preconcentration of amino acids using a sol-gel coated column coupled to a conventional UV/vis detector. Extraction, stacking and focusing techniques are used in the preconcentration procedures. Sol-gel coatings are created by using N-Octadecyldimethyl[3(trimethoxysilyl)proply] ammonium chloride (C18-TMS) in the coating sol solutions. The resulting sol-gel coating carries a positive charge. For extraction, the pH of the samples is properly adjusted to impart a net negative charge to amino acids. A long plug of the sample is then passed through the sol-gel coated capillary to facilitate extraction via electrostatic interaction between the positively charged sol-gel coating and the negatively charged amino acid molecules. The focusing of the extracted amino acids is accomplished through desorption of the extracted amino acid molecules carried out by local pH change. The described procedure provides 150,000-fold enrichment effect for alanine.
REFERENCES:
Guo et al. (“Hydrolytically stable amino-silica glass coating material for manipulation of the elecroosmotic flow in capillary electrophoresis,” Journal of Chromatography A, 744 (1996) 17-29).
Hsieh et al. (“Electroosmotic flow controllable coating on a capillary surface by a sol-gel process for capillary electrophoresis,” Journal of Chromatography A, 952 (2002) 255-266).
Michael et al.; Sensitivity Enhancement for Capillary Electrophoresis; Analytical Chemistry; vol. 65, No. 10; May 15, 1993.
Chien et al.; Sample Stacking of an Extremely Large Injection Volume in High-Performance Capillary Electrophoresis; American Chemical Society; 1992.
Chien et al.; On-Column Sample Concentration Using Field Amplification in CZE; Analytical Chemistry; vol. 64, No. 8; Apr. 15, 1992.
Shihabi; Stacking in Capillary Zone Electrophoresis; Elsevier Science B.V.; 2000. Journal of Chromatography A, 902, pp. 107-117.
Shihabi; Stacking and Discontinuous Buffers in Capillary Zone Electrophoresis; Wiley-VCH Verlag Gmbh, 69451 Weinheim 2000.
Chun et al.; Protein Analysis with Large Volume Sample Stacking with an Electrosmotic Flow Pump: A Potential Approach for Proteomics; Elsevier Science B.V. 2001.
Locke et al.; Techniques for the Optimization of Proteomic Strategies Based on Head Column Stacking Capillary Electrophoresis; Analytical Chemistry; vol. 72; No. 13; Jul. 1, 2000.
Britz-McKibbin et al.; Selective Focusing of Catecholamines and Weakly Acidic Compounds by Capillary Electrophoresis Using . . . ; Analytical Chemistry; vol. 72, No. 6; Mar. 15, 2000.
Sample Injections; Instrumental Considerations; p. 106-111. Publication date and publisher not provided by Applicant.
Wei et al.; One-Step Concentration of Analytes Based on Dynamic Change in pH in Capillary Zone Electrophoresis; Analytical Chemistry; vol. 74, No. 5; Mar. 1, 2002.
Britz-McKibbin et al.; On-Line Focusing of Flavin Derivatives Using Dynamic pH Junction-Sweeping Capillary Electrophoresis . . . ; Analytical Chemistry; vol. 74, No. 15; Aug. 1, 2002.
Nunez et al.; Sample Stacking with Matrix Removal for the Determination of Paraquat, Diquat and Difenzoquat in Water by Capillary Electrophoresis; Elsevier Science B.V.; 2001.
Hjerten et al.; Adaptation of the Equipment for High-Performance Electrophoresis to Isoelectric Focusing; Elsevier Science B.V.; 1985.
Shen et al.; High-Efficiency Capillary Isoelectric Focusing of Peptides; Analytical Chemistry; vol. 72, No. 9, May 1, 2000.
Spanik et al.; Use of Full-Column Imaging Capillary Isoelectric Focusing for the Rapid Determination of the Operating Conditions in the Preparative . . . ; Elsevier Science B.V.; 2002.
Clarke et al.; Capillary Isoelectric Focusing-Mass Spectrometry: Analysis of Protein Mixtures From Human Body Fluids; Biomedical Chromatography; 2002.
Hiraoka et al.; One-Step Capillary Isoelectric Focusing of the Proteins in Cerebrospinal Fluid and Serum of Patients with Neurological Disorders; Journal of Chromatography; 2002.
Cifuentes et al.; Capillary Isoelectric Focusing of Erythropoietin Glycoforms and its Comparison with Flat-Bed Isoelectric Focusing . . . ; Journal of Chromatography; 1999.
Righetti et al.; Study of Haptoglobin-Hemoglobin Complexes by Titration Curves, Capillary Electrophoresis and Capillary Isoelectric Focusing; Journal of Chromatography; 1997.
Martin et al.; Displacement Electrophoresis; Roy. Soc. Lond.; 1970.
Tu et al.; Determination of Nitrate in Seawater by Capillary Zone Electrophoresis with Chloride-Induced Sample Self-Stacking; Journal of Chromatography; 2002.
Church et al.; Transient Isotachophoretic-Electrophoretic Separations of Lanthanides with Indirect Laser-Induced Fluorescence Detection; Analytical Chemistry; 1998.
Toussaint et al.; Enantiomeric Separation of Clenbuterol by Transient Isotachophoresis-Capillary Zone Electrophoresis-UV Detection New . . . ; Journal of Chromatography; 2000.
Shihabi; Transient Pseudo-Isotachophoresis for Sample Concentration in Capillary Electrophoresis; Electrophoresis; p. 1612-1617;2002.
Shihabi et al.; Insulin Stacking for Capillary Electrophoresis; Journal of Chromatography; 1998.
Kameoka et al.; A Polymeric Microfluidic Chip for CE/MS Determination of Small Molecules; Analytical Chemistry; 2001.
Deng et al.; Chip-Based Quantitative Capillary Electrophoresis/Mass Spetrometry Determination of Drugs in Human Plasma; Analytical Chemistry; 2001.
Deng et al.; Chip-Based Quantitative Capillary Electrophoresis/Mass Spectrometry Determination of Drugs in Human Urine; Analytical Chemistry; 2001.
Yang et al.; Sample Stacking in Laboratory-On-A-Chip Devices; Journal of Chromatography; 2001.
Lichtenberg et al.; Sample Preconcentration by Filed Amplification Stacking for Microchip-Based Capillary Electrophoresis; Electrophoresis; p. 258-271; 2001.
Quirino et al.; Approaching a Million-Fold Sensitivity Increase in Capillary Electrophoresis with Direct Ultraviolet Detection: Cation-Selective Exhaustive . . . ; Analytical Chemistry; 2000.
Quirino et al.; Sweeping of Analyte Zones in Electrokinetic Chromatography; Analytical Chemistry; 1999.
Quirino et al.; Exceeding 5000-Fold Concentration of Dilute Analytes in Micellar Electrokinetic Chromatography; www.sciencemag.org; vol. 282; Oct. 16, 1998.
Kim et al.; On-line Sample Concentration in Micellar Electrokinetic Chromatography Using Cationic Surfactants; Journal of Chromatography; 2001.
Quirino et al.; On-line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography; Journal of Chromatography; 1997, pp. 255-267, vol. 791.
Quirino et al.; Sweeping with an Enhanced Electric Field of Neutral Analyte Zones in Electrokinetic Chromatography; High Resol. Chromatography; 1999 22, (7) 367-372.
Quirino et al.; On-line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography 5 Field-Enhanced Sample Injection . . . ; Analytical Chemistry; 1998, vol. 70, No. 9.
Quirino et al.; On-line Concentration of Neutral Analytes for Micellar Electrokinetic Chromatography; Journal of Chromatography; 1997, 781 (1997) 119-128.
Palmer et al.; Stacking Neutral Analytes in Capillary Electrokinetic Chromatography with High-Salt Sample Matrixes; Analytical Chemistry; 2000, 2000, 72, 1941-1943.
Palmer et al.; A Universal Concept for Stacking Neutral Analytes in Micellar Capillary Electrophoresis; Analytical Chemistry; 1999, 71, 1679-1687.
Palmer et al.; Electrokinetic Injection for Stacking Neutral Analytes in Capillary and Microchip Electrophoresis; Analytical Chemistry; 2001, vol. 73, No. 4, Feb. 15, 2001.
Cao et al.; Stacking Lonizable Analytes in a Sample Matrix with High Salt by a Transient Moving Chemical Reaction Boundary Method in Capillary Zone . . . ; Analytical Chemistry; 2002.
Strausbauch et al.; Mechanism of Peptide Separations by Solid Phase Extraction Capillary Electrophoresis at Low pH; Analytical Chemistry; 1996, 1996, 68, 306-314.
Veraart et al.; At-Line Solid-Phase Extraction Coupled to Capillary Electrophoresis: Determination of Amphoteric Compounds in Biological . . . ; J. High Resol. Chromatography; 1999, 1999, 22, (3
Fries David P.
Li Wen
Malik Abdul
Hopen Anton J.
Noguerola Alex
Smith & Hopen , P.A.
University of South Florida
LandOfFree
Method of using sol-gel coatings for on-line... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of using sol-gel coatings for on-line..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of using sol-gel coatings for on-line... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3995670