Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...
Reexamination Certificate
2006-11-28
2006-11-28
Gakh, Yelena (Department: 1743)
Chemistry: analytical and immunological testing
Nuclear magnetic resonance, electron spin resonance or other...
C436S086000
Reexamination Certificate
active
07141432
ABSTRACT:
The present invention discloses eight new reduced dimensionality (RD) triple resonance nuclear magnetic resonance (NMR) experiments for measuring chemical shift values of certain nuclei in a protein molecule. The RD 3DHA,CA,(CO),N,HN NMR experiment and the RD 3DH,C,(C-TOCSY—CO),N,HN NMR experiment are designed to yield “sequential” connectivities, while the RD 3DHαβ,Cαβ,CO,HA NMR experiment and the RD 3DHαβ,Cαβ,N,HN NMR experiment provide “intraresidue” connectivities. The RD 3DH,C,C,H—COSY NMR experiment, the RD 3DH,C,C,H-TOCSY NMR experiment, and the RD 2DH,C,H—COSY NMR experiment allow one to obtain assignments for aliphatic and aromatic side chain chemical shifts, while the RD 2DHB,CB,(CG,CD),HD NMR experiment provide information for the aromatic side chain chemical shifts. In addition, a method of conducting suites of RD triple resonance NMR experiments for high-throughput resonance assignment of proteins and identification of the location of secondary structure elements are disclosed.
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Gakh Yelena
Nixon & Peabody LLP
The Research Foundation of State University of New York
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