Method of using mobile priming sites for DNA sequencing

Details Method of using mobile priming sites for DNA sequencing Method of using mobile priming sites for DNA sequencing

1994-07-14

1996-04-30

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 6, 435810, 536 231, 536 243, C12P 1934, C12Q 168, C07H 2102, C07H 2104

Patent

active

055124585

ABSTRACT:
Doubled-stranded oligonucleotide molecules for use in providing enchaned sequencing of DNA, comprising: a first stand having 1) a priming site; 2) a labeling region contiguous with the priming site, wherein the labeling region consists essentially of at least six identical nucleotides; and 3) a cohesive end contiguous with the labeling region, having a nucleotide sequence complementary to a nucleotide sequence generated by the action of a restriction enzyme; and a second strand annealed to the labeling region of the first stand, but not overlapping with the cohesive end. A method of DNA gene sequencing, involving the use of mobile priming sites for DNA polymerases in DNA sequencing, is also disclosed.

REFERENCES:
patent: 5426039 (1995-06-01), Wallace et al.
Friend, Tim, "High-tech labs shorten the path to new genes," U.S.A. Today, Mar. 22, 1994; p. 5D.
Lamerdin, Jane E. and Carrano, Anthony V., "Automated Fluorescence-Based Restriction Fragment Analysis," BioTechniques, vol. 15, No. 2 (1993); pp. 294-302.
Unrau, Paul and Deugau, Kenneth V., "Non-cloning amplification of specific DNA fragments from whole genomic DNA digests using DNA `indexers`", Gene, 145(1994); pp. 163-169.
Vooijs, M., Yu, L. C., Tkachuk, D. Pinkel, D. Johsnon, D., Gray, J. W.; "Libraries for Each Human Chromosome, Construction From Sorter-enriched Chromosomes by Using Linker-Adaptor PCR", Am. J. Hum. Genet., 52:586-597, 1993.
Wesley, Cedric S., Ben, Mathew, Martin, Krietman, Hagag, Nabil, Eanes, Walter F., "Cloning regions of the drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer", Nucleic Acids Research, vol. 18, No. 3; pp. 599-603.

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