Method of using isotropic, porous-wall polymeric membrane, hollo

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435161, 435171, 435174, 435183, 435284, 435813, C12P 2100, C12N 900, C12M 300

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active

044422067

ABSTRACT:
Hollow fiber reactors for growing microbial cells. Isotropic hollow fibers are supported in a housing inoculated with cells. Nutrient medium passing through the lumen undergoes a pressure drop resulting in radial convective flow: the nutrient medium flows outwardly from the lumen into the surrounding area adjacent the entry port and fluid surrounding the hollow fiber flows into the lumen adjacent the exit port. With the efficient distribution of nutrients and removal of product, high cell densities are achieved providing for high product yields per unit reactor volume.

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Kan et al., "Urocanic Acid Production Using Whole Cells Immobilized on a Hollow-Fiber Reactor", Biotech. & Bioeng., vol. 20, pp. 271-320, 1978.
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Porter, M. C., Biotechnology and Bioengineering Symposium No. 3, "Applications of Membranes to Enzyme Isolation and Purification", 1972, pp. 115-130.

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