Method of using gold nanorods-siRNA complexes

Nanotechnology – Nanostructure – Nanoparticle

Reexamination Certificate

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C514S04400A

Reexamination Certificate

active

08035016

ABSTRACT:
Provided are methods and compositions for inhibiting expression of one or more target genes. The compositions contain RNA polynucleotides that can inhibit expression of a target gene via RNA interference (RNAi) electrostatically complexed with surface functionalized gold nanorods (GNRs). The RNA polynucleotides are not covalently bound to the surface functionalized GNRs. The method involves inhibiting expression of a target gene in an individual. The method is performed by administering to the individual an effective amount of a composition containing surface functionalized GNRs electrostatically complexed with RNA polynucleotides, such as siRNA, that can inhibit expression of the target gene via RNAi. The siRNA is not covalently bound to the surface functionalized GNRs.

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Roy et al.; Non-viral gene transfection nanoparticles: Function and applications in brain; Nanomedicine, Jun. 2008, vol. 4, No. 2; pp. 89-97.
Niidome et al.; Preparation of primary amine-modified gold nanoparticles and their transfection ability into cultivated cells; Chem. Commun., Sep. 7, 2004, vol. 17; pp. 1978-1979.
Ding et al.; Gold Nanorods Coated with Multilayer Polyelectrolyte as Contrast Agents for Multimodal Imaging; J. Phys. Chem., 2007, vol. 111, No. 34; pp. 12552-12557.

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