Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy
Patent
1992-11-03
1994-10-04
Tung, T.
Chemistry: electrical and wave energy
Processes and products
Processes of treating materials by wave energy
204403, 204415, G01N 27327, G01N 27404
Patent
active
053523482
ABSTRACT:
Disclosed is a method of assaying a high concentration of a substance in a liquid sample with a polarographic cell. The cell contains an electrode assembly that includes a reference electrode and a hydrogen peroxide sensor electrode having a laminated membrane covering the liquid sample contacting face of the sensor electrode. The laminated membrane includes an outer membrane permeable to the substance and oxygen, an inner membrane permeable to hydrogen peroxide and located adjacent the face of the sensor electrode, and an enzyme layer between the inner and outer membrane; the enzyme in the enzyme layer can oxidize the substance to generate hydrogen peroxide. The method includes contacting the outer membrane with the liquid sample; permitting the substance and oxygen in the liquid sample to pass through the outer membrane to contact the enzyme layer so that where the substance is oxidized to generate hydrogen peroxide; permitting the generated hydrogen peroxide to pass through the inner membrane to contact the sensor electrode; and ensuring that the supply of oxygen in the enzyme layer relative to the supply of glucose is sufficient to produce an equilibrium concentration of hydrogen peroxide, which generates a steady state response at the sensor electrode proportional to the concentration of the substance in the liquid sample.
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Winarta Handani
Young Chung Chang
Nova Biomedical Corporation
Tung T.
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