Method of using colored phosphorylating reagents

Details Method of using colored phosphorylating reagents Method of using colored phosphorylating reagents

1987-08-18

1993-10-12

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Acyclic carbon bonded directly to three benzene rings or to...

544218, 558168, 536 553, 536 253, C07F 909, C07F 9141, C07F 922, C07H 1104

Patent

active

052527603

ABSTRACT:
A method of phosphorylating a nucleoside or an oligonucleotide chain having a free 2', 3' or 5' hydroxyl moiety is provided. The method involves the use of a phosphorylating reagent which is selected such that the extent of phosphorylation can be monitored colorimetrically, easily and accurately. The phosphorylating reagent contains an aromatic species such as a dimethoxytrityl group that is cleavable with acid and colorimetrically detectable upon release. Examples of phosphorylating reagents which are useful in conjunction with the disclosed method include ##STR1##

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