Method of using as a selectable marker a nucleic acid containing

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800200, 536 236, 536 241, 4351723, 435 691, 4353201, 435419, C12N 1529, C12N 1582, A01H 400, A01H 500

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active

060255412

ABSTRACT:
The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences.
AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.

REFERENCES:
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McElroy, D., Foreign Gene Expression in Transgenic Cereals, Tibtech, Feb. 1994 vol. 12.
Christensen, A.H., Sequence Analysis and Transcriptional Regulation by Heat Shock of Polyubiquitin Transcripts from Maize, Plant Molecular Biology 12:619-632 (1989).
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