Method of using a reference control composition for...

Details Method of using a reference control composition for... Method of using a reference control composition for...

2007-04-03

2007-04-03

Wallenhorst, Maureen M. (Department: 1743)

Chemistry: analytical and immunological testing

Biological cellular material tested

C436S010000, C436S164000, C436S166000

Reexamination Certificate

active

10960370

ABSTRACT:
Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.

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