Electricity: electrical systems and devices – Safety and protection of systems and devices – With specific voltage responsive fault sensor
Patent
1999-05-18
2000-06-20
Leja, Ronald W.
Electricity: electrical systems and devices
Safety and protection of systems and devices
With specific voltage responsive fault sensor
323220, H02H 314
Patent
active
060784903
ABSTRACT:
A method is provided for treating materials, especially organic materials, with pulsed electrical fields, wherein the method includes the step of applying an agile pulse sequence having at least three pulses to a material, wherein the agile pulse sequence has one, two, or three of the following characteristics: (1) at least two of the at least three pulses differ from each other in pulse amplitude; (2) at least two of the at least three pulses differ from each other in pulse width; and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses. When biological cells are treated to form pores in an electroporation procedure, the induced pores are sustained for a relatively long period of time, and viability of the biological cells is maintained.
REFERENCES:
patent: 4459541 (1984-07-01), Fielden et al.
patent: 5444358 (1995-08-01), Delepaut
Cyto Pulse Sciences, Inc.
Laughlin, Jr. James H.
Leja Ronald W.
Towsend Marvin S.
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