Electrolysis: processes – compositions used therein – and methods – Electrolytic material treatment – Organic
Patent
1995-12-08
2000-01-04
Mayekar, Kishor
Electrolysis: processes, compositions used therein, and methods
Electrolytic material treatment
Organic
4351736, 4351735, C12N 1300
Patent
active
060106131
ABSTRACT:
A method is provided for treating materials, especially organic materials, with pulsed electrical fields, wherein the method includes the step of applying an agile pulse sequence having at least three pulses to a material, wherein the agile pulse sequence has one, two, or three of the following characteristics: (1) at least two of the at least three pulses differ from each other in pulse amplitude; (2) at least two of the at least three pulses differ from each other in pulse width; and (3) a first pulse interval for a first set of two of the at least three pulses is different from a second pulse interval for a second set of two of the at least three pulses. When biological cells are treated to form pores in an electroporation procedure, the induced pores are sustained for a relatively long period of time, and viability of the biological cells is maintained.
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King Alan D.
Walters Richard E.
Cyto Pulse Sciences, Inc.
Mayekar Kishor
Towsend Marvin S.
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