Method of treating biological cells and/or their cell...

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or...

Reexamination Certificate

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C435S070200, C435S070210, C435S173400, C435S173500, C435S173600, C435S173900, C435S184000, C435S212000, C435S219000, C435S346000, C436S548000

Reexamination Certificate

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10348831

ABSTRACT:
A method is described for treating biological cells and/or their cell components with electrical fields in a reaction medium, in which an inhibitor is added to the reaction medium to counteract the action of enzymes that break down protein.

REFERENCES:
patent: 4578167 (1986-03-01), Schoner
patent: 5403585 (1995-04-01), Malfroy-Camine et al.
G. Fuhr, U. Zimmermann and S. G. Shirley, “Electromanipulation of Cells,” Cell Motion in Time-Varying Fields: Principles and Potential, Chapter 5, pp. 259-328, CRC Press, Buca Raton, 1996.
U. Zimmermann and C. Pilwat “Electric Field Simulated Fusion: Increased Field Stability of Cells Induced by Pronase,” Naturwissenschaften 68, p. 577, Springer Verlag, 1981.

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