Surgery – Blood vessel or graft preparation
Patent
1991-03-06
1992-07-21
Wax, Robert A.
Surgery
Blood vessel or graft preparation
623 1, 427 40, 427 41, 424 93, 435 1, 4352407, 435240241, A61F 204, A61F 202, B05D 306, C12N 506
Patent
active
051319071
ABSTRACT:
A novel implantable prosthesis for implantation in human patients is disclosed which comprises a synthetic substrate and a Type IV/V collagen surface layer seeded with a confluent monolayer of autologous endothelial cells. In the preferred embodiment, a base layer of interstitial collagen is adhered to the substrate. The Type IV/V collagen surface layer is provided in a laminate comprising an intermediate layer of interstitial collagen which is covalently bound to the aforementioned base layer. A cross linking agent, such as glutaraldehyde, is utilized to covalently bind the interstitial collagen base layer to the substrate, and the collagen intermediate layer to the base layer. The cross linking agent is subsequently deactivated with a soluble peptide, such as lysine. Autologous endothelial cells, such as microvascular endothelial cells derived from adipose tissue, are then seeded at high densities on the pretreated graft material. Those cells adhere in suitable percentage within times compatible with most vascular surgical procedures, and, even more importantly, flatten on that surface to assume the appearance of a natural, cobblestone morphology. The subject surface is resistant to shear stress, and yields good results when tested as a vena cava implant in a dog.
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Jarrell Bruce E.
Williams Stuart K.
Low Christopher S. F.
Thomas Jefferson University
Wax Robert A.
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