Method of transforming monocotyledonous seed

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium

Reexamination Certificate

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C800S278000

Reexamination Certificate

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07544858

ABSTRACT:
Provided is a method of transforming a monocotyledon by usingagrobacterium. There is provided a method of transforming a monocotyledon by usingagrobacteriumcontaining a desired recombinant gene. This transforming method comprises the steps of sowing a culture medium containing a plant growth factor with a monocotyledonous seed, conducting culturing for 1 to 3 days so as to effect germination and infecting the seed withagrobacterium. This method makes it feasible to rapidly transform monocotyledons, including a rice plant.

REFERENCES:
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patent: WO01/06844 (2001-02-01), None
Park, H-D., et al. (1995). “Construction of Binary Vectors for the Rice TransformationUsing a Rice Actin Promoter and Replication Origin of pTi12 Isolated from Agrobacterium tumefaciensKU12.” J. Plant. Biol. 38(4):365-371. Jan. 1, 1995.
Toki, S. et al., “Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice”,The Plant Journal, 47:969-976 (2006).
The European Office Action/Supplemental European Search Report from European Patent Application EP 05 721 519.6, search report dated Feb. 23, 2009, 7 pages (2009).
Dale, P.J. et al., “Agroinfection of Wheat: Inoculation of In Vitro Growth Seedlings and Embryos”,Plant Science, 63:237-245 (1989).

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