Method of transformation of nocardia lactamdurans

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352523, 435872, C12N 121, C12N 1563, C12N 1576

Patent

active

057563268

ABSTRACT:
A method is disclosed for the high efficiency transformation of species of the genus Nocardia with DNA molecules. DNA vectors for the transformation of genes into Nocardia as well as recombinant Nocardia host cells expressing recombinant genes are disclosed.

REFERENCES:
patent: 4791064 (1988-12-01), Fare et al.
Chen, Carton W., et al., Cloning and expression of a DNA sequence conferring cephamycin C production, Biotechnology, vol. 6, Oct. 1988 pp. 1222-1224. (cumulative).
Ginther, Charles L, et al., Sporulation and the Production of Serine Protease and Cephamycin C by Streptomyces lactamdurans, Antimicrobial Agents and Chemotherapy, vol. 15, No. 4, Apr. 1979, pp. 522-526. (cumulative).
Garcia-Dominquez et al., Antimicro. Agents and Chemoth. 35(1): 44-52 (1991).
R. Coque, Juan Jose, et al., Genes for a .beta.--lactamase, a penicillin-binding protein and a transmembrane protein are clustered with the cephamycin biosynthetic genes in Nocardia lactamdurans, The EMBO Journal, vol. 12 No. 2, pp. 631-639 1993. (cumulaative).
Castro, Jose M., et al., Regulation of .alpha.-aminoadiply-cysteinyl-valine, isopenicillin N synthetase, isopenicillin N isomerase and deacetoxycephalosponn C synthetase by nitrogen sources in Streptomyces lactamdurans Appl. Microbiol. Biotechnol. 22: pp. 32-40 1985 (cumulative).
Janssen and Bibb, Derivatives of pUC18 that have Bglll sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies, Gene, 124 pp. 133-134 1993. (cumulative).
Kieser and Melton, Plasmid plJ699, a multi-copy positive-selection vector for Streptomyces, Gene, 65, pp. 83-91 1988. (cumulative).
R. Coque, Juan Jose, et al., A Gene Encoding Lysine 6-Aminotransferase, Which Forms the .beta.-Lactam Precursor .alpha.-Aminoadipic Acid, is Located in the Cluster of Cephamycin Biosynthetic Genes in Nocardia lactamdurans, Journal of Bacteriology vol. 173, No. 19, pp. 6258-6264 1991. (cumulative).
Matsushima and Baltz, Efficient Plasmid Transformation of Streptomyces ambofaciens and Streptomyces fradiae Protoplasts, Journal of Bacteriology, vol. 163, No. 1, pp. 180-185 1985. (cumulative).
Thompson, Charles et al., Cloning of Antibiotic Resistance and Nutritional Genes in Streptomycetes, Journal of Bacteriology, vol., 151, No. 2, pp. 668-677, 1982. (cumulative).
Madon and Hutter, Transformation System for Amycolatopsis (Nocardia) mediterranei: Direct Transformation of Mycelium with Plasmid DNA, Journal of Bacteriology, vol. 173, No. 20, pp. 6325-6331, 1991. (cumulative).
Lal, Rup, et al., Construction of a Hybrid Plasmid Capable of Replication in Amycolatopsis mediterranei, Applied and Environmental Microbiology, vol. 57, No. 3, pp. 665-671, 1991. (cumulative).
Garcia-Dominguez, Modesta, Efficient Plasmid Transformation of the .beta.-Lactam Producer Streptomyces clavuligerus, Applied and Enviromental Microbiology, vol. 53, No. 6, pp. 1376-1381, 1987. (cumulative).
Murray, I.A., et al., Nucleotide sequence of the chloramphenicol acetyltransferase gene of Streptomyces acrimycini, Gene, vol 85 pp. 283-291 1989. (cumulative).
Criado, Luis M., et al., The pab gene of Steptomyces griseu, encoding p-aminobenzoic acid synthase, is located between genes possibly involved in candicidin biosynthesis, Gene, vol. 126 pp. 135-139, 1993. (cumulative).
Albertini, Alessandra, et al., On the Formation of Spontaneous Deletions: The importance of Short Sequence Homologies in the Generation of Large Deletions, Cell, vol. 29, pp. 319-328, 1982.
Santamaria, Ramon et al., High-Frequency Transformation of Brevibacterium lactofermentum Protoplasts by Plasmid DNA, Journal of Bacteriology, vol. 162, No. 1, pp. 463-467, 1985. (cumulative).
MacNeil, Douglas, Characterization of a Unique Methyl-Specific Restriction System in Streptomyces avermitilis, Journal of Bacteriology, vol. 170, No. 12, 1988. (cumulative).
Lechevalier, Hubert; Nocardioform Actinomycetes, Bergey's Manual of Systematic Bacteriology, vol. 4, Williams, Sharpe, and Holt (eds) Williams & Wilkins (pubs.) Baltimore, Md.) Section 26, pp. 2348-2361 1989. (cumulative).
Powell, Ian, et al., A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation, Applied and Environmental Microbiology, pp. 655-660, 1988. (cumulative).
Lechevalier, M.P., et al., Two New Genera of Nocardioform Actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov., International Journal of Systematic Bacteriology, vol. 36, No. 1, pp. 29-37, 1986. (cumulative).
Wesseling and Lago, Strain Improvement by Genetic Recombination of Cephamycin Producers, Nocardia lactamdurans and Streptomyces griseus, Developments Ind. Microbiol., vol. 22, pp. 641-651 1981. (cumulative).
Stapley, E.O., et al., Cephamycins, a New Family of B-Lactam Antibiotics, Antimicrobial Agents and Chemotherapy, vol. 2, No. 3, pp. 122-131 1972. (cumulative).
Coque, J.J., et al., The Cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nacardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum, Molecular Microbiology, vol. 5, (5) pp. 1125-1133, 1991. (cumulative).
Clayton and Bibb, Streptomyces promoter-probe plasmids that utilise the xylE gene of Pseudomonas putida, Nucleic Acids Research, vol. 18, No. 4, pp. 1077, 1989.
Kieser, Tobias, et al., plJ101, a Multi-Copy Broad Host-Range Streptomyces Plasmid: Functional Analysis and Development of DNA Cloning Vectors, Mol. Gen. Genet. vol. 185, pp. 223-238, 1982. (cumulative).
Martin, J.F., Cloning Systems in Amino Acid-producing Corynebacteria, Biotechnology, vol. 5, pp. 137-146, 1987. (cumulative).
Dunican and Shivnan, High Frequency Transformation of Whole Cells of Amino Acid Producing Coryneform Bacteria Using High Voltage Electroporation, Biotechnology, vol. 7, pp. 1067-1070, 1989. (cumulative).
Martin, Juan F., Clusters of genes for the biosynthesis of antibiotics: regulatory genes and overproduction of Pharmaceuticals, Society for Industrial Microbiology, vol. 9, pp. 73-90, 1992. (cumulative).
Santamaria, Ramon, et al., Characterization of an Endogenous Plasmid and Development of Cloning Vectors and a Transformation System in Brevibacterium lactofermentum, Journal of General Microbiology, vol. 130, pp. 2237-2246, 1984. (cumulative).
Castro, Jose M, et al., Purification and Characterization of the Isopenicillin N Synthase of Streptomyces lactamdurans, Journal of General Microbiology, vol., 134, pp. 133-141, 1988. (cumulative).
Cortes, Jesus, et al., Purification and Characterization of a 2-Oxoglutarate-linked ATP-independent Deacetoxycephalosporin C Synthase of Streptomyces lactamdurans, Journal of General Microbiology, vol. 133, pp. 3165-3174, 1987. (cumulative).
Laiz, Leonila, et al., Purification and Characterization of the isopenicillin N epimerase from Nocardia lactamdurans, Journal of General Microbiology, vol. 136, pp. 663-671, 1990.
Katz, Edward, et al., Cloning and Expression of the Tyrosinase Gene from Streptomyces antibioticus in Streptomyces lividans, Journal of General Microbiology, vol. 129, pp. 2703-2714, 1983(cumulative).
Kieser, Tobias, Factors Affecting the Isolation of CCC DNA from Streptomyces lividans and Escherichia coli, Plasmid, vol. 12, pp. 19-36, 1984, (cumulative).
Coque, Juan Jose, et al., Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans: the organization of the cephamycin gene cluster differs from that in Steptomyces clavuligerus, Mol. Gen. Genet, vol. 236, pp. 453-458, 1993. (cumulative).
Matushima et al. Efficient Transformation of Amycolatopsis orientalis ( Nocardia orientalis) Protoplasts by Streptamyces Plasmids, J. of Bacteriology, May 1987 pp. 2298-2300. (cumulative).
Maniatis et al. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory p. 4, 1982, (cumulative).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of transformation of nocardia lactamdurans does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of transformation of nocardia lactamdurans, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of transformation of nocardia lactamdurans will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1959020

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.