Method of transferring substance into cell

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S461000, C435S470000, C435S471000

Reexamination Certificate

active

07927874

ABSTRACT:
There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.

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Okubo et al., “DNA Introduction into Living Cells by Water Droplet Impact with an Electrospray Process,”Angewandte Chemie(International Edition), 2008, vol. 47, No. 8, pp. 1429-1431.
Chen et al., “A novel approach for introducing bio-materials into cells,”Journal of Nanoparticle Research, 2000, vol. 2, pp. 133-139.
Ikemoto, “Gene introduction into cell by electrospray,” The 52ndMeeting of the Japan Society of Applied Physics and Related Societies,Extended Abstracts, 2005, p. 1451.
First Office Action dated Jun. 9, 2010 corresponding (Chinese Application No. 2007/80022741.8), Chinese and English translation from the Republic of China Patent Office.
Second Office Action dated Dec. 6, 2010 corresponding (Chinese Application No. 2007/80022741.8), Chinese and English translation from the PRC Patent Office.

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