Method of transduction of cells with retroviral vectors

Details Method of transduction of cells with retroviral vectors Method of transduction of cells with retroviral vectors

1995-05-26

1997-07-15

Guzo, David

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

C12N 510, C12N 1564

Patent

active

056482515

ABSTRACT:
The present invention relates to an efficient method, termed spin transduction, of transducing cells with retroviral vectors. Retroviral particles, suspended in a pharmaceutically acceptable carrier, are contacted with the cells to be transduced, and the mixture is centrifuged for a sufficient period of time to permit transduction. This method leads to a 3- to 15-fold increase in transduction efficiency, depending on the type of cells transduced.

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