Method of thioacylation peptide sequencing with alcoholysis of t

Details Method of thioacylation peptide sequencing with alcoholysis of t Method of thioacylation peptide sequencing with alcoholysis of t

1988-03-10

1989-09-05

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 92, G01N 2125, G01N 3368

Patent

active

048638706

ABSTRACT:
A sequencing method which exploits the thioacylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After sufficient time to insure quantitative coupling and removal of excess reagent, the N-thioacyl polypeptide is subjected to cleavage by acid which affords a 2-substituted-5(4H)-thiazolinone of the N-terminal amino acid. Subsequent addition of a large excess of an aliphatic primary or secondary alcohol, either directly to the cleavage acid or after its removal, yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and subsequent detection by contemporary methods of high pressure liquid chromatography.

REFERENCES:
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patent: 4548904 (1985-10-01), Kent et al.
patent: 4652530 (1987-03-01), Rothman
Previero et al, Chemical Abstracts, vol. 84, No. 21, Abstract No. 147268m, 5/24/76.
Tsugita et al. Chemical Abstracts, vol. 106, No. 11, Abstract No. 81238r, 3/16/87.

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