Method of thawing cryopreserved cells

Details Method of thawing cryopreserved cells Method of thawing cryopreserved cells

1996-09-25

1999-04-20

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435325, 436 18, A01N 102, C12N 500, G01N 3100

Patent

active

058957456

ABSTRACT:
The invention features a method of processing cryopreserved cells by thawing and equilibrating the cells at warm temperatures (e.g., between 30.degree. C. and 43.degree. C). Either the cell suspension in the cryoprotective medium is thawed to a temperature between 35.degree. C. and 43.degree. C. or the cryoprotective medium is equilibrated with a culture medium at a temperature between 35.degree. C. and 43.degree. C., or both steps are carried out at the warm temperatures. By thawing and equilibrating the cryopreserved cells at warm temperatures, the viability, (especially after 3 hours of culture), and metabolic activity (i.e., diazepam metabolism) of the cells can be improved over traditional cold cell processing (i.e., at temperatures of between 2.degree. C. and 8.degree. C.).

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