Method of testing for the presence of Salmonella serotypes expre

Details Method of testing for the presence of Salmonella serotypes expre Method of testing for the presence of Salmonella serotypes expre

1995-05-25

1996-04-23

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 735, 43524027, 530350, 5303871, 5303884, 5303895, 5303911, 5303913, G01N 3353, C07K 14255, C07K 1600

Patent

active

055102417

ABSTRACT:
A method of testing for the presence of Salmonella serotypes S. enteritidis and S. dublin is provided. Novel monoclonal antibodies are used to detect the presence of an epitope specific for these serotypes in cultures which have been grown on selected media which enhance the expression of said epitope in fimbrial sites. Test kits utilizing the antigen or its epitopic parts, antibodies and/or the media are further provided.

REFERENCES:
Manual of Clinical Microbiology, 4th Ed. Lennette edition (American Society for Microbiology, Washington DC) 1985, p. 1062.
American Type Culture Collection Catalogue of Bacteria and Phages, 17th Ed., 1989, pp. 295, 317, 342.
Handbook of Microbiological Media: Atlas et al, (CRC Press, Boca Raton) 1993, pp. 459, 801.
Feutrier et al, "Purification and Characteristics of Fimbriae from Salmonella enteridites", J. Bacteriol., 168(1): 221-227 (Oct. 1986).
Feutier et al, "Cloning and Expression of a Salmonella enteriditis Fimbrin Gene in Escherichia coli", J. Bacteriol., 170(9): 4216-4222 (Sep. 1988).
Thorns et al, "Detection of a Novel Fimbrial Structure on the Surface of Salmonella enteriditis by Using a Monoclonal Antibody", J. Clin. Microbiol., 28(11): 2409-2414 (Nov. 1990).

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