Chemistry: analytical and immunological testing – For preexisting immune complex or auto-immune disease – Antinuclear
Patent
1981-10-16
1985-01-15
Padgett, Benjamin R.
Chemistry: analytical and immunological testing
For preexisting immune complex or auto-immune disease
Antinuclear
436513, 436518, 436543, 436804, 436811, 436823, 436824, 436825, 435 6, 435 7, 435 19, G01N 3354, G01N 3356, C12Q 144, C12Q 168
Patent
active
044938993
ABSTRACT:
A method is provided for testing for particular antibodies in the serum of a patient. The antibodies may be those of systemic lupus erythematosus and may constitute IgG and IgM immunoglobulins. The IgG and IgM immunoglobulin may be individually labeled radioactively.
An antigen (such as DNA) may be attached to a support such as sepharose. The attachment may be facilitated as by irradiation with ultraviolet light. The DNA may be single stranded or double stranded. When double-stranded DNA is used, single-stranded portions in the double strands may be removed as by a suitable enzyme.
The particular antibodies may be attached to the antigen such as the supported DNA. An assay may then be provided to determine the attachment of the particular antibodies to the supported DNA. When the particular antibodies constitute immunoglobins of systemic lupus erythematosus, the assay may actually provide a determination of the amounts of the IgG and IgM immunoglobulins individually attached to the single-stranded and double-stranded DNA.
REFERENCES:
patent: 3992517 (1976-11-01), Lowke et al.
patent: 4189464 (1980-02-01), Blumberg et al.
patent: 4234563 (1980-11-01), Rippe
patent: 4251514 (1981-02-01), Rippe
patent: 4267270 (1981-05-01), Stout
patent: 4273756 (1981-06-01), Ling et al.
Heinzerling, R. H. et al., J. Investigative Dermatology, vol. 75(3), pp. 224-227 (1980).
Miller, Thomas E., Arthritis-Rheumatism, vol. 24(4), pp. 602-610 (4-1981).
Okudaira, Hirokazu et al., J. Immunological Methods, vol. 41, (pp. 201-213 (3-1981).
Hashimoto, H. et al., Nippon Rinsho, vol. 37(2), pp. 452-462 (1979).
Aarden, Lucien A. et al., Immunological Methods, vol. II, pp. 75-82 (1981).
Adam, C. et al., J. of Immunological Methods, vol. 27, pp. 133-143 (1979).
Fish, Falk et al., Fed. Proceedings, vol. 39(3), p. 472 (1980) and Arthritis & Rheumatism, vol. 24(3), pp. 534-543 (1981).
Harrington, John T. et al., Arthritis & Rheumatism, vol. 19(4), p. 801 (1976).
Lange et al., Clinical-Experimental Immunology, vol 25(2), pp. 191-198 (1976).
Smith et al., Journal of Laboratory-Clinical Medicine, vol. 98, (3), pp. 425-436 (1981).
Smith Lloyd H.
Teplitz Raymond L.
City of Hope
Moskowitz M.
Padgett Benjamin R.
Roston Ellsworth R.
Schwartz Charles H.
LandOfFree
Method of testing for particular antibodies in the serum of a pa does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of testing for particular antibodies in the serum of a pa, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of testing for particular antibodies in the serum of a pa will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1481482