Method of testing expression of CA125 antigen in cultured ovaria

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 721, 436 63, 436 64, 436813, G01N 33574, G01N 3353

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active

058719415

ABSTRACT:
A screening test for identifying individuals having a predisposition to develop ovarian cancer. The test involves measuring the phenotypic expression of the CA125 antigen in overtly normal ovarian surface epithelium (OSE) cells propagated in vitro. The OSE cells are preferably passaged repeatedly prior to measuring for the expression of the CA125 antigen. The test is based on the finding that OSE cells obtained from individuals having a family history of ovarian and/or breast cancer retain sub-populations of CA125 expressing cells for longer time periods in vitro than OSE cells derived from individuals not having a such a family history. The test provides an alternative means of identifying and monitoring women at risk of developing ovarian cancer, including women with hereditary ovarian cancer syndromes and women likely to develop sporadic (non-familial) ovarian cancer.

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