Method of terminating isocitrate dehydrogenase reaction in an an

Details Method of terminating isocitrate dehydrogenase reaction in an an Method of terminating isocitrate dehydrogenase reaction in an an

1986-04-22

1988-05-03

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 12, C12Q 132

Patent

active

047420014

ABSTRACT:
Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of ATP and/or a chelating agent in a system wherein NAD.sup.+ formed from NADH is reproduced into NADH in the conjoint presence of an isocitrate, metallic ions such as magnesium or manganese ions, and isocitrate dehydrogenase in assaying a substance by means of a reaction of NADH to NAD.sup.+.

REFERENCES:
patent: 3331752 (1967-07-01), Struck
patent: 4427771 (1984-01-01), Misaki et al.
patent: 4622296 (1986-11-01), Yamanishi et al.

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