Method of terminating isocitrate dehydrogenase reaction

Details Method of terminating isocitrate dehydrogenase reaction Method of terminating isocitrate dehydrogenase reaction

1992-12-11

1993-11-02

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435184, 435190, 435 12, C12Q 132, C12N 944, C12N 904

Patent

active

052582868

ABSTRACT:
Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of a chelating agent in a system wherein NADP.sup.+ formed from NADPH is reproduced into NADPH in the conjoint presence of an isocitrate, metallic ions such as magnesium or manganese ions, and isocitrate dehydrogenase in assaying a substance by means of a reaction of NADPH to NADP.sup.+.

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