Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-07-12
1995-10-24
Martinell, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 9153, 4351723, 935 16, 935 78, C12N 1500, C12N 1509
Patent
active
054609410
ABSTRACT:
The present invention relates to a method of forming a three-stranded DNA molecule wherein each strand of the three-stranded DNA molecule is hybridized (that is, non-covalently bound) to at least one other strand of the three-stranded DNA molecule. The method comprises:
contacting a recombination protein with a double-stranded DNA molecule and with a single-stranded DNA molecule sufficiently complementary to one strand of the double-stranded DNA molecule to hybridize therewith, which contacting is effected under conditions such that the single-stranded DNA molecule hybridizes to the double-stranded molecule so that the three stranded DNA molecule is formed.
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Camerini-Otero Carol S.
Camerini-Otero Rafael D.
Ferrin Lance J.
McIntosh Margaret
Martinell James
The United States of America as represented by the Secretary of
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