Method of target enrichment and amplification

Details Method of target enrichment and amplification Method of target enrichment and amplification

2007-05-08

2007-05-08

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S091100, C435S091510, C435S091520, C536S024300, C536S024200

Reexamination Certificate

active

10654281

ABSTRACT:
The presently claimed invention provides methods and kits for amplifying a target sequence from within a nucleic acid population. The presently claimed invention provides selection probes which are complementary to at least a portion of said target sequence and mechanisms for adding a probe sequence to the 3′ end of a target sequence that is hybridized to a selection probe. The added 3′ probe sequence and a probe sequence added at the 5′ end of the target by adaptor ligation allow for selective amplification of the target sequence.

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Nikolai Lisitsyn, et al., “Cloning the Differences Between Two Complex Genomes”, Research Article, Science, vol. 259, Feb. 12, 1993, pp. 946-951.

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