Method of synthesizing sulfurized oligonucleotide analogs with t

Details Method of synthesizing sulfurized oligonucleotide analogs with t Method of synthesizing sulfurized oligonucleotide analogs with t

1990-01-12

1992-11-24

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Phosphorus esters

536 27, 536 553, 568 13, 568 14, 568 15, 548113, 548119, C07F 904, C07H 2100

Patent

active

051663872

ABSTRACT:
A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiuram disulfide as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.

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