Method of synethesizing sulfurized oligonucleotide analogs

Details Method of synethesizing sulfurized oligonucleotide analogs Method of synethesizing sulfurized oligonucleotide analogs

1990-04-20

1992-09-29

Lacey, David L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, 514 12, 514 44, 435 6, 4351721, C07H 1512, C07H 1700, A01N 4304, C12Q 168

Patent

active

051515105

ABSTRACT:
A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.

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