Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Patent
1998-10-27
2000-11-07
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
435377, 435375, 435383, C12N 500
Patent
active
061435600
ABSTRACT:
The present invention relates to a method of synchronizing epithelial cells into G.sub.0 phase, more specifically, a method of synchronizing epithelial cells into G.sub.0 phase characterized by culturing the cells in a synthetic medium for 5 days or more, wherein said synthetic medium is a medium with the lowered concentration of bovine fetal serum 5% or less and said epithelial cells is bovine mammary gland cells. Further, the present invention relates to a method of culturing cells characterized by using G.sub.0 phase synchronized cells obtained by the present invention, and a method of screening a related to differentiation and/or maturation of said cells by using G.sub.0 phase synchronized cells. The present invention is useful for easily synchronizing epithelial cells into G.sub.0 phase and for screening a factor related to differentiation and/or maturation of epithelial cells.
REFERENCES:
Jouvenot et al. Uterine epithelial cells in primary culture: a model system to study cell proliferation. Cell. Mol. Biol. 36(4): 421-427, 1990.
Sia et al. Quiescent mammary epithelial cells have reduced connexin43 but maintain a high level of gap junction intercellular communication. Dev. Genet. 24(1-2): 111-122, 1999.
Larsson et al. Immediate effects of serum depletion on dissociation between growth in size and cell division in proliferating 3T3 cells. J. Cell. Physiol. 127(2): 267-73, May, 1986.
Lehmann et al. Response of different mammary epithelial cell lines to a mammary derived growth factor. Biomed. Biochim. Acta 48 (1): 143-151, 1989.
Liu et al. Estimation of PCNA mRNA stability in cell cycle by a serum-deprivation method. Journal of Cellular Biochemistry 57: 641-646, 1995.
Zavizion et al. Effect of mammary-derived growth inhibitor on proliferation of MAC-T bovine mammary epithelial cells. J. Dairy Science 76(12): 3721-3726, Dec. 1993.
Zetterberg et al. Kinetic analysis of regulatory events in G.sub.1 leading to proliferation or quiescence of Swiss 3T3 cells. Proc. Natl. Acas. Sci. USA 82: 5365-5369, Aug. 1985.
Imamura Mio
Itagaki Yasuharu
Tanimoto Morimasa
Lankford , Jr. Leon B.
Snow Brand Milk Products Co. Ltd.
LandOfFree
Method of synchronizing epithelial cells into G.sub.0 phase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of synchronizing epithelial cells into G.sub.0 phase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of synchronizing epithelial cells into G.sub.0 phase will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1639573