Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector
Reexamination Certificate
2005-04-19
2005-04-19
Smith, Lynette R. F. (Department: 1645)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
C424S234100, C424S252100, C435S069100, C435S410000, C435S420000, C435S069300, C435S006120, C435S470000, C536S023100, C536S023200, C536S023700, C536S023710, C514S04400A
Reexamination Certificate
active
06881411
ABSTRACT:
This invention satisfies needs in the art by providing intimin, the EnterohemorrhagicEscherichia coli(EHEC) adherence protein, alone or as a fusion protein with one or more other antigens, expressed by transgenic plants and the use of those plants as vehicles for stimulating a protective immune response against EHEC and the one or more other antigens. Various plant species are transformed to protect various animal species and also humans against EHEC, against pathogens expressing intimin-like proteins, and against pathogens expressing any of the one or more other antigens to which intimin may be fused.The eae gene encoding intimin, a functional portion thereof, or a recombination that encodes a fusion protein is put under the control of a constitutive plant promoter in a plasmid and the plasmid is introduced into plants by the type of transformation appropriate for the particular plant species. The engineered plants expressing intimin or the intimin fusion protein are then fed to animals and/or humans to elicit the production of antibodies, which protect the animals/humans against EHEC colonization and infection, and against pathogens expressing the one or more other antigens and any cross-reactive antigens. The invention may also be practiced by expressing the intimin or intimin fusion protein in other host organisms such as bacteria, yeast, and fungi.
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McKee and O'Brien, Abstract B
McKee Marian L.
O'Brien Alison D.
Stewart, Jr. C. Neal
Wachtel Marian R.
Finnegan Henderson Farabow Garrett & Dunner L.L.P.
Henry M. Jackson Foundation for the Advancement of Military Medi
Portner Ginny Allen
Smith Lynette R. F.
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