Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1989-03-24
1991-12-17
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 791, 435 18, 435 21, 536 27, 935 77, 935 78, C12Q 168, C12Q 134, C12Q 142, G01N 3353
Patent
active
050734830
ABSTRACT:
A method of staining a nucleic acid sequence which is fixed on a solid support is described wherein an enzymatic system which fixes this sequence is employed to detect it by non-radioactive labelling, the enzymatic system being subsequently reacted with chromogenic substrate to produce a colored product. A developer solution is used comprising a sulfur organic compound constituting the substrate which is adapted to be hydrolized by the enzyme to form an organic thiol, and a metallic compound, for example a gold compound. The metallic compound is soluble and stable in aqueous solution and can react with the organic thiol to form a metal-organic compound complex having a characteristic color which precipitates in situ, which metal-organic complex subsequently may be decolorized with a reducing agent to thus allow reuse of the solid support. A set, or kit, of reactants for carrying out the method also is disclosed.
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Chemical Abstract, vol. 101:1984, Abstract No. 16726f.
Colloidal Gold: Principles and Methods & Applications, vol. 2, Academic Press, N.Y., Chap 19, (1989), pp. 380-396.
Moeremans, G, (1984), Sensitive Visualization of Ag-Ab React in Dot and Blot Immune Ov . . . , J. Immunol. Meth 74:353-360.
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Surekr Latzko (1984), Visualization of Antigenic Prot. Bloted onto Nitrocellulose Using Immun Au-Bioch. Bioph. Res. Com 121(1), 284-289.
Bioprobe Systems
Escallon Miguel H.
Wax Robert A.
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