Method of staining monocytes and compositions thereof

Details Method of staining monocytes and compositions thereof Method of staining monocytes and compositions thereof

1990-11-01

1992-04-21

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

424 3, 436 63, 436174, 436805, 435 29, 435 34, G01N 130

Patent

active

051067447

ABSTRACT:
This invention is directed to the cytology of blood, bone marrow and lymph node cells and to the method of differentiating, identifying and enumerating monocytes among a plurality of cells of hematopoietic origin. More specifically, the invention is directed to stained cells and to the use of a cationic sulfur-containing azo dye capable of staining monocytes a bright red-violet color. The dye is a water soluble quaternary azo dye identified in the Colour Index as Basic Blue 54 (C.I. 11052). The stained monocytes obtained by the process of this invention have color characteristics which permit the identification and differentiation of said cells by use of various instruments including, for example, an image analyzer, microscope, photo microscope, and the like.

REFERENCES:
patent: 3906120 (1975-09-01), Geating
patent: 4020006 (1977-04-01), Parker
patent: 4070495 (1978-01-01), Berger et al.
patent: 4581223 (1986-04-01), Kass
patent: 4714606 (1987-12-01), Kass
patent: 4810487 (1989-03-01), Kass
patent: 4996040 (1991-02-01), Kass

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