Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...
Reexamination Certificate
2007-12-18
2007-12-18
Lankford, Jr., Leon B. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving fixed or stabilized, nonliving microorganism,...
C435S034000
Reexamination Certificate
active
10005753
ABSTRACT:
A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.
REFERENCES:
patent: 4877459 (1989-10-01), Cockrell et al.
patent: 5135736 (1992-08-01), Anderson et al.
patent: 5245008 (1993-09-01), Dickhardt et al.
patent: 5366730 (1994-11-01), Kohnert et al.
patent: 5439801 (1995-08-01), Jackson
patent: 5578320 (1996-11-01), Janoff et al.
patent: 5668011 (1997-09-01), Jackson
patent: 5773592 (1998-06-01), Mills
patent: 5912219 (1999-06-01), Carrie et al.
patent: 0 882 983 (1998-12-01), None
patent: 1136563 (2001-09-01), None
patent: 826066 (1959-12-01), None
patent: WO 9810273 (1998-03-01), None
Bates, R. “Amine buffers for pH control” Annals NY Acad. Sci. (1961) 92: 341-356.
Derwent Publications Ltd., “New Methine Dyes are Capable of Being Effectively Excited with Economical Helium-Neon Laser Light Source with Resultant Stronger Fluorescent Intesity”, JP 2000 273330A, (Fuji Photo Film Co. Ltd.), Oct. 3, 2000 Abstract.
G. P. James et al., “Urinary Nitrite and Urinary-Tract Infection”, Am J. Clinical Pathol, vol. 70, 1978, pp. 671-678.
Y. Nakagawa, et al., “Characteristic Bleaching Profiles of Cyanine Dyes Depending on Active Oxygen Species in the Controlled Fenton Reaction”, Biological and Pharmaceutical Bulletin, vol. 16, No. 11, 1993, pp. 1061-1064.
H. Okada, et al., “Detection of Significant Bacteriuria by Automated Urinalysis Using Flow Cytometry”, Journal of Clinical Microbiology, vol. 38, No. 8, Aug. 2000, pp. 2870-2872.
S. Smith et al., “Detection of Urinary Tract Infections by Reduction of Nitroblue Tetrazolium: Technical Note”, Kidney International, vol. 54. No. 4, Oct. 1998, pp. 1331-1336.
Ikeuchi Yoshiro
Inoue Junya
Kawashima Yasuyuki
Sakai Yasuhiro
Hanley Susan
Lankford , Jr. Leon B.
Sysmex Corporation
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